EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54521
![Flow Cytometry: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]](https://resources.bio-techne.com/images/products/EpCAM-TROP1-Antibody-rMOC-31-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54521-img0003.jpg "Flow Cytometry: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]")
![Western Blot: EpCAM/TROP1 Antibody (rMOC-31)Azide and BSA Free [NBP2-54521]](https://resources.bio-techne.com/images/products/EpCAM-TROP1-Antibody-rMOC-31-Azide-and-BSA-Free-Western-Blot-NBP2-54521-img0004.jpg "Western Blot: EpCAM/TROP1 Antibody (rMOC-31)Azide and BSA Free [NBP2-54521]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rMOC-31
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
Immunogen
Recombinant full-length human EpCAM/TROP1 protein (Uniprot: P16422 )
Epitope
aa 27-59
Reactivity Notes
Does not react with Rat, Canine or Feline.
Localization
Cell surface
Specificity
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of EpCAM.EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (EpCAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of recombinant monoclonal antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53329).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
Western Blot: EpCAM/TROP1 Antibody (rMOC-31)Azide and BSA Free [NBP2-54521]
Western Blot: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521] - Western blot analysis of human lung tissue lysate using EpCAM/TROP1 antibody (rMOC-31).![Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]](https://resources.bio-techne.com/images/products/EpCAM-TROP1-Antibody-rMOC-31-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54521-img0002.jpg "Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]")
Immunocytochemistry/ Immunofluorescence: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]
Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521] - Confocal immunofluorescence analysis of MCF-7 cells using labeled in Green. Red Dot is used to label the nucleus red.![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]](https://resources.bio-techne.com/images/products/EpCAM-TROP1-Antibody-rMOC-31-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54521-img0001.jpg "Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]")
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521]
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free [NBP2-54521] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with Ep-CAM Recombinant Mouse Monoclonal Antibody (rMOC-31).Applications for EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Western Blot
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: EpCAM/TROP1
EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).
References
1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255
2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764
3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956
4. Uniprot (P16422)
5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018
Long Name
Epithelial Cell Adhesion Molecule
Alternate Names
17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1
Gene Symbol
EPCAM
Additional EpCAM/TROP1 Products
Product Documents for EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
Product Specific Notices for EpCAM/TROP1 Antibody (rMOC-31) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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