EphA2 Antibody (M049)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23315
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M049
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EphA2 Antibody (M049)
Immunogen
Clone (M049) was generated from a recombinant protein that included the extracellular region of human EphA2 protein. [UniProt# P29317]
Specificity
Clone M049 mouse monoclonal antibody detects a 125kDa protein on SDS-PAGE "Native" immunoblots of human NCI-H2052 and MDA-MB-231 carcinomas, as well as the EphA2 C-terminal 60 kDa fragment in human A431 and A549. The antibody detects native EphA2, as well as aldehyde-fixed EphA2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for EphA2 Antibody (M049)
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (M049) [NBP3-23315] -
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (M049) [NBP3-23315] - Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (NBP3-23315). The antibody was detected using goat anti-mouse DyLight(R) 594.
Western Blot: EphA2 Antibody (M049) [NBP3-23315] -
Western Blot: EphA2 Antibody (M049) [NBP3-23315] - Western blot of human MDA-MB-231 breast carcinoma (lane 1), A431 epidermoid carcinoma (lane 2), NCI-H2052 epithelioid mesothelioma (lane 3), and A549 lung carcinoma (lane 4). The blot was probed with mouse monoclonal anti-EphA2 (NBP3-23315) at 1:1000.
ELISA: EphA2 Antibody (M049) [NBP3-23315] -
ELISA: EphA2 Antibody (M049) [NBP3-23315] - Representative Standard Curve using mouse monoclonal anti-EphA2 (NBP3-23315) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.Applications for EphA2 Antibody (M049)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: EphA2
Alternate Names
Eck, Myk2, Sek2
Gene Symbol
EPHA2
Additional EphA2 Products
Product Documents for EphA2 Antibody (M049)
Product Specific Notices for EphA2 Antibody (M049)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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