EphA4 [p Tyr779] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23330

Novus Biologicals, part of Bio-Techne
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NBP3-23330
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphA4 Antibodies »

Product Summary for EphA4 [p Tyr779] Antibody

Immunogen

EphA4 (Tyr-779) synthetic peptide corresponds to amino acids surrounding phosphorylated tyrosine 779 in human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and is conserved well in most other EphA family members, but has less conservation in EphB family members. [UniProt# P54764]

Modification

p Tyr779

Specificity

This antibody was cross-adsorbed to unrelated phosphotyrosine peptides before affinity purification using EphA4 (Tyr-779) peptide. The purified antibody detects a 120 kDa band corresponding to EphA4 in western blots of HUVEC cells treated with pervanadate.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EphA4 [p Tyr779] Antibody

EphA4 [p Tyr779] Antibody

Western Blot: EphA4 [p Tyr779] Antibody [NBP3-23330] -

Western Blot: EphA4 [p Tyr779] Antibody [NBP3-23330] - Western blot analysis of human umbilical vein endothelial cells untreated (lanes 1, 3, 5, & 7) or treated with pervanadate (1 mM) for 30 min. (lanes 2, 4, 6, & 8). The blot was probed with anti-EphA4 (N-terminal region) (lanes 1 & 2), anti-EphA4 (Tyr-779) (lanes 3 & 4), anti-EphA4 (Tyr-602) (lanes 5 & 6), or anti-EphA4 (C-terminal region) (lanes 7 & 8).

Applications for EphA4 [p Tyr779] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EphA4

Eph receptor A4 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Long Name

Eph Receptor A4

Alternate Names

Cek8, Hek8, Sek1, Tyro1

Gene Symbol

EPHA4

Additional EphA4 Products

Product Documents for EphA4 [p Tyr779] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EphA4 [p Tyr779] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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