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EphB1 Antibody (4G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00002047-M01

Novus Biologicals, part of Bio-Techne
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H00002047-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EPHB1 (NP_004432.1, 221 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACT

Specificity

EPHB1 - EPH receptor B1 (4G6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EphB1 Antibody (4G6)

Western Blot: EphB1 Antibody (4G6) [H00002047-M01]

Western Blot: EphB1 Antibody (4G6) [H00002047-M01]

Western Blot: EphB1 Antibody (4G6) [H00002047-M01] - Analysis of EPHB1 expression in transfected 293T cell line by EPHB1 monoclonal antibody (M01), clone 4G6.Lane 1: EPHB1 transfected lysate (Predicted MW: 109.9 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: EphB1 Antibody (4G6) [H00002047-M01] - Detection limit for recombinant GST tagged EPHB1 is 0.03 ng/ml as a capture antibody.

Applications for EphB1 Antibody (4G6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphB1

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq]

Long Name

Eph Receptor B1

Alternate Names

Cek6, Elk, Hek6, Net

Gene Symbol

EPHB1

UniProt

Additional EphB1 Products

Product Documents for EphB1 Antibody (4G6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EphB1 Antibody (4G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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