EphB4 Antibody (5B5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47434

Novus Biologicals, part of Bio-Techne
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NBP1-47434
NBP1-47434-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B5

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all EphB4 Antibodies »

Product Specifications

Immunogen

Purified recombinant extracellular fragment of human EphB4 fused with hIgGFc tag expressed in HEK293 cell line.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphB4 Antibody (5B5) - BSA Free

Western Blot: EphB4 Antibody (5B5)BSA Free [NBP1-47434]

Western Blot: EphB4 Antibody (5B5)BSA Free [NBP1-47434]

Western Blot: EphB4 Antibody (5B5) [NBP1-47434] - Western blot analysis using anti-EphB4 monoclonal antibody against extracellular domain of human EphB4(aa16-539).
Immunocytochemistry/ Immunofluorescence: EphB4 Antibody (5B5) - BSA Free [NBP1-47434]

Immunocytochemistry/ Immunofluorescence: EphB4 Antibody (5B5) - BSA Free [NBP1-47434]

Immunocytochemistry/Immunofluorescence: EphB4 Antibody (5B5) [NBP1-47434] - Confocal immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using anti-EphB4 monoclonal antioby(green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.

Applications for EphB4 Antibody (5B5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1: 500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which binds of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands.Ephs and ephrins play a significant role in angiogenesis.

Long Name

Eph Receptor B4

Alternate Names

Htk, Mdk2, Myk1, Tyro11

Gene Symbol

EPHB4

Additional EphB4 Products

Product Documents for EphB4 Antibody (5B5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphB4 Antibody (5B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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