EphB6 Antibody (5D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00002051-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EphB6 Antibodies »

Product Specifications

Immunogen

EPHB6 (NP_004436, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

EPHB6 - EPH receptor B6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EphB6 Antibody (5D8)

Western Blot: EphB6 Antibody (5D8) [H00002051-M03]

Western Blot: EphB6 Antibody (5D8) [H00002051-M03]

Western Blot: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of EPHB6 expression in NIH/3T3 (Cat # L018V1).
Immunohistochemistry-Paraffin: EphB6 Antibody (5D8) [H00002051-M03]

Immunohistochemistry-Paraffin: EphB6 Antibody (5D8) [H00002051-M03]

Immunohistochemistry-Paraffin: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of monoclonal antibody to EPHB6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml
Western Blot: EphB6 Antibody (5D8) [H00002051-M03]

Western Blot: EphB6 Antibody (5D8) [H00002051-M03]

Western Blot: EphB6 Antibody (5D8) [H00002051-M03] - Analysis of EPHB6 expression in PC-12 (Cat # L012V1).
View 1 more images

Applications for EphB6 Antibody (5D8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using H00002051-M03 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EphB6

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands.

Long Name

Eph Receptor B6

Alternate Names

Hep, Mep

Entrez Gene IDs

2051 (Human)

Gene Symbol

EPHB6

OMIM

602757 (Human)

UniProt

NP_004436

Additional EphB6 Products

Product Documents for EphB6 Antibody (5D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphB6 Antibody (5D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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