Ephrin-A5 Antibody (1F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00001946-M01

Novus Biologicals, part of Bio-Techne
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H00001946-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ephrin-A5 Antibodies »

Product Specifications

Immunogen

EFNA5 (NP_001953, 114 a.a. ~ 203 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSEKFQLFTPFSLGFEFRPGREYFYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGEN

Specificity

EFNA5 - ephrin-A5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ephrin-A5 Antibody (1F12)

Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01]

Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01]

Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01] - EFNA5 monoclonal antibody (M01), clone 1F12. Analysis of EFNA5 expression in IMR-32.
Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01]

Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01]

Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01] - analysis of EFNA5 expression in NIH/3T3 ( Cat # L018V1 ).
ELISA: Ephrin-A5 Antibody (1F12) [H00001946-M01]

ELISA: Ephrin-A5 Antibody (1F12) [H00001946-M01]

ELISA: Ephrin-A5 Antibody (1F12) [H00001946-M01] - Detection limit for recombinant GST tagged EFNA5 is approximately 0.03ng/ml as a capture antibody.

Applications for Ephrin-A5 Antibody (1F12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ephrin-A5

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Alternate Names

AL-1, EFL-5, EFNA5, EphrinA5, LERK-7, RAGS

Entrez Gene IDs

1946 (Human)

Gene Symbol

EFNA5

OMIM

601535 (Human)

UniProt

NP_001953 (Human)

Additional Ephrin-A5 Products

Product Documents for Ephrin-A5 Antibody (1F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ephrin-A5 Antibody (1F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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