Ephrin-A5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58251

Novus Biologicals, part of Bio-Techne
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NBP2-58251
NBP2-58251-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ephrin-A5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: FYISSAIPDNGRRSCLKLKVFVRPTNSCMKTIGVHDRVFDVNDKVENSLEPADDTVHESAEPSRGENAAQ

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28169277).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ephrin-A5 Antibody

Immunocytochemistry/ Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251]

Immunocytochemistry/ Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251]

Immunocytochemistry/Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.

Applications for Ephrin-A5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

Reported in scientific literature (PMID 28169277)
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 1 using NBP2-58251 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ephrin-A5

Ephrin A5, a member of the ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.

Alternate Names

AL-1, EFL-5, EFNA5, EphrinA5, LERK-7, RAGS

Gene Symbol

EFNA5

Additional Ephrin-A5 Products

Product Documents for Ephrin-A5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ephrin-A5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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