Ephrin-B1 [p Tyr317] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-275
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (100%), Chicken (100%), Human (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ephrin-B1 conjugated to KLH. Accession # P28693
Reactivity Notes
Tested in Rat. Mouse reactivity reported in scientific literature (PMID: 21539827).
Modification
p Tyr317
Specificity
Specific for endogenous levels of the ~46 kDa Ephrin-B1 protein phosphorylated at Tyr317. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Ephrin-B1 [p Tyr317] Antibody
![Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275]](https://resources.bio-techne.com/images/products/EPH-Receptor-B1-[p-Tyr317]-Antibody-Western-Blot-NB300-275-img0001.jpg "Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275]")
Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275]
Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.Applications for Ephrin-B1 [p Tyr317] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:1000
Application Notes
This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa. Immunofluorescence (PMID: 21530605)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ephrin-B1
Alternate Names
Cek5-L, EFL-3, EFNB1, ELK-L, EphrinB1, LERK-2, STRA-1
Gene Symbol
EFNB1
Additional Ephrin-B1 Products
Product Documents for Ephrin-B1 [p Tyr317] Antibody
Product Specific Notices for Ephrin-B1 [p Tyr317] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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