epithelial Sodium Channel beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84844

Novus Biologicals, part of Bio-Techne
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NBP1-84844
NBP1-84844-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all epithelial Sodium Channel beta Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRGEKYCNNRDFPDWAHCYSDLQMSVAQRETCIGMCKESCNDTQYKMTISMADWPSEASEDWIFHVLS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (88%). Human reactivity reported in scientific literature (PMID: 26230923).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for epithelial Sodium Channel beta Antibody

Western Blot: epithelial Sodium Channel beta Antibody [NBP1-84844]

Western Blot: epithelial Sodium Channel beta Antibody [NBP1-84844]

Western Blot: epithelial Sodium Channel beta Antibody [NBP1-84844] - Analysis in control (vector only transfected HEK293T lysate) and sCNN1B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: epithelial Sodium Channel beta Antibody [NBP1-84844]

Immunohistochemistry-Paraffin: epithelial Sodium Channel beta Antibody [NBP1-84844]

Immunohistochemistry-Paraffin: epithelial Sodium Channel beta Antibody [NBP1-84844] - Staining of human small intestine shows cytoplasmic positivity in glandular cells.

Applications for epithelial Sodium Channel beta Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 26230923). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: epithelial Sodium Channel beta

SCNN1B is a subunit of the epithelial sodium channel, ENaC. ENac has high sodium selectivity, low conductance, and amiloride sensitivity. The functional channel of ENaC is composed of at least 3 subunits, alpha (SCNN1A), beta (SCNN1B), and gamma (SCNN1G). The 3 subunits show sequence similarities to one another, indicating descent from a common ancestral gene. Each encodes a protein containing 2 transmembrane domains, with intracellular amino and carboxyl termini. ENaC in the kidney, lung and colon plays an essential role in trans- epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium re-uptake in the distal nephron of the kidney, thus regulating blood pressure. Mutations in beta or gamma subunit of the epithelial sodium channel (ENaC) have been found to cause a hereditary form of human hypertension, Liddle syndrome.

Long Name

Amiloride-sensitive sodium channel subunit beta

Alternate Names

Beta-ENaC, Beta-NaCH, ENaCB, SCNEB, SCNN1B

Gene Symbol

SCNN1B

Additional epithelial Sodium Channel beta Products

Product Documents for epithelial Sodium Channel beta Antibody

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Certificate of Analysis

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Product Specific Notices for epithelial Sodium Channel beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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