EPLIN Antibody (1B7)
Novus Biologicals, part of Bio-Techne | Catalog # H00051474-M06
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1B7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
LIMA1 (AAH01247, 1 a.a. ~ 598 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MENCLGESRHEVEKSEISENTDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKPLSPDSRASSLSESSPPKAMKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYCNNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQLANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGSSGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQSTSVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKGETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFTTQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE
Specificity
Reacts with LIM domain and actin binding 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for EPLIN Antibody (1B7)
![Western Blot: EPLIN Antibody (1B7) [H00051474-M06]](https://resources.bio-techne.com/images/products/EPLIN-Antibody-1B7-Western-Blot-H00051474-M06-img0001.jpg "Western Blot: EPLIN Antibody (1B7) [H00051474-M06]")
Western Blot: EPLIN Antibody (1B7) [H00051474-M06]
Western Blot: EPLIN Antibody (1B7) [H00051474-M06] - Western Blot detection against Immunogen (91.89 KDa) .![Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06]](https://resources.bio-techne.com/images/products/EPLIN-Antibody-1B7-Immunohistochemistry-Paraffin-H00051474-M06-img0004.jpg "Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06]")
Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06]
Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06] - Analysis of monoclonal antibody to LIMA1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
Sandwich ELISA: EPLIN Antibody (1B7) [H00051474-M06] - Detection limit for recombinant GST tagged LIMA1 is 0.3 ng/ml as a capture antibody.
Applications for EPLIN Antibody (1B7)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This antibody is reactive against recombinant protein in Western Blot and ELISA. Also works in IHC-Paraffin.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EPLIN
Alternate Names
1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3
Entrez Gene IDs
51474 (Human)
Gene Symbol
LIMA1
UniProt
Additional EPLIN Products
Product Documents for EPLIN Antibody (1B7)
Product Specific Notices for EPLIN Antibody (1B7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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