EPLIN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87947

Novus Biologicals, part of Bio-Techne
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NBP1-87947
NBP1-87947-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EPLIN Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ISENSYSLDDLEIGPGQLSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKIHKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDS

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 24135282).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EPLIN Antibody

EPLIN Antibody

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] -

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] -Analysis in human rectum and liver tissues using NBP1-87947 antibody. Corresponding LIMA1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947]

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947]

Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human liver, pancreas, rectum and testis using Anti-LIMA1 antibody NBP1-87947 (A) shows similar protein distribution across tissues to independent antibody NBP2-38673 (B).
Western Blot: EPLIN Antibody [NBP1-87947]

Western Blot: EPLIN Antibody [NBP1-87947]

Western Blot: EPLIN Antibody [NBP1-87947] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
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Applications for EPLIN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EPLIN

This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]

Alternate Names

1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3

Entrez Gene IDs

51474 (Human)

Gene Symbol

LIMA1

UniProt

Q9UHB6 (Human)

Additional EPLIN Products

Product Documents for EPLIN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EPLIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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