EPS15R Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-88149
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 814 and 864 of human epidermal growth factor receptor pathway substrate 15-like 1 using the numbering given in entry NP_067058.1 (GeneID 58513).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EPS15R Antibody
Western Blot: EPS15R Antibody [NB100-88149]
Western Blot: EPS15R Antibody [NB100-88149] - Detection of Human EPS15L1 on HeLa whole cell lysate using NB100-88149. EPS15L1 was also immunoprecipitated by another rabbit anti-EPS15L1 antibody.Western Blot: EPS15R Antibody [NB100-88149] -
Western Blot: EPS15R Antibody [NB100-88149] - EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(D) HeLa cells stably expressing GFP-tagged wild-type EPS15, EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR & for control tubulin (TUB) analyzed by immunoblotting with specific antibodies. Experiments quantified in Fiji & the EPS15 levels plotted as fold-increase compared to controls. Data mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for EPS15R Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EPS15R
Alternate Names
epidermal growth factor receptor pathway substrate 15-like 1, epidermal growth factor receptor substrate 15-like 1, epidermal growth factor receptor substrate EPS15R, Eps15R, Eps15-related protein
Entrez Gene IDs
58513 (Human)
Gene Symbol
EPS15L1
UniProt
Additional EPS15R Products
Product Documents for EPS15R Antibody
Product Specific Notices for EPS15R Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...