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EPS15R Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-88149

Novus Biologicals, part of Bio-Techne
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NB100-88149

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 814 and 864 of human epidermal growth factor receptor pathway substrate 15-like 1 using the numbering given in entry NP_067058.1 (GeneID 58513).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EPS15R Antibody

Western Blot: EPS15R Antibody [NB100-88149]

Western Blot: EPS15R Antibody [NB100-88149]

Western Blot: EPS15R Antibody [NB100-88149] - EPS15 depletion stabilizes ESCRT components HRS, STAM and TSG101. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/13841) licensed under a CC-BY license.
Western Blot: EPS15R Antibody [NB100-88149]

Western Blot: EPS15R Antibody [NB100-88149]

Western Blot: EPS15R Antibody [NB100-88149] - Detection of Human EPS15L1 on HeLa whole cell lysate using NB100-88149. EPS15L1 was also immunoprecipitated by another rabbit anti-EPS15L1 antibody.
EPS15R Antibody

Western Blot: EPS15R Antibody [NB100-88149] -

Western Blot: EPS15R Antibody [NB100-88149] - EPS15 is targeted via a SPOP/SPOPL binding consensus motif.(D) HeLa cells stably expressing GFP-tagged wild-type EPS15, EPS15S744-S746A or the EPS15K793R mutants from a doxycycline-inducible promoter transfected as indicated (+) with control siRNA or siRNA depleting SPOPL. The levels of EPS15-GFP, EGFR & for control tubulin (TUB) analyzed by immunoblotting with specific antibodies. Experiments quantified in Fiji & the EPS15 levels plotted as fold-increase compared to controls. Data mean ± SEM, N = 4. *p≤0.05. Note that SPOPL depletion does not further increase the levels of both EPS15 mutants. Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for EPS15R Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EPS15R

EPS15L1 (epidermal growth factor receptor substrate 15-like 1), also known as EPS15R (EPS15-related), is structurally related to the EPS15 protein, a protein involved in the receptor-mediated endocytosis of the EGF receptor. EPS15L1 and EPS15 both contain an EH (Eps15 homology) domain, a protein-protein interaction domain found in several proteins that participate in endocytosis, vesicle transport, and signal transduction.

Alternate Names

epidermal growth factor receptor pathway substrate 15-like 1, epidermal growth factor receptor substrate 15-like 1, epidermal growth factor receptor substrate EPS15R, Eps15R, Eps15-related protein

Entrez Gene IDs

58513 (Human)

Gene Symbol

EPS15L1

Additional EPS15R Products

Product Documents for EPS15R Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EPS15R Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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