Epsin-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30171

Novus Biologicals, part of Bio-Techne
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NBP3-30171
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Epsin-2 Antibodies »

Product Specifications

Immunogen

Between 150 and 200

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Epsin-2 Antibody

Epsin-2 Antibody

Immunoprecipitation:Epsin-2 AntibodyNBP3-30171] -

Immunoprecipitation:Epsin-2 AntibodyNBP3-30171] -Detection of human EPN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPN2 antibody NBP3-30171 (lot NBP3-30171-1) used for IP at 6 µg per reaction. EPN2 was also immunoprecipitated by rabbit anti-EPN2 antibody BL21886. For blotting immunoprecipitated EPN2, NBP3-30171 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Epsin-2 Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Epsin-2

Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimately in cancer. Two novel EGF receptor substrates designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15, have been described. Epsin is a 90 kDa binding partner to Eps15. Both epsin and Eps15 have an ubiquitous tissue distribution but are concentrated in presynaptic nerve terminals specialized for the Clathrin-mediated endocytosis of synaptic vesicles. Disruption of epsin function blocks Clathrinmediated endocytosis. Epsin along with its binding partner Eps15 is proposed to be involved in the assistance of Clathrin coat rearrangement during Clathrin coated pit invagination. Epsin 2 and epsin 2a are also associated with Clathrin-mediated endocytosis and are enriched in the brain in the peri-Golgi region.

Alternate Names

Eps15 binding protein, EPS-15-interacting protein 2, epsin 2, epsin-2, KIAA1065EHB21

Gene Symbol

EPN2

Additional Epsin-2 Products

Product Documents for Epsin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Epsin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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