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EQTN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90869

Novus Biologicals, part of Bio-Techne
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NBP1-90869
NBP1-90869-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SCESQYSVNPELATMSYFHPSEGVSDTSFSKSAESSTFLGTTSSDMRRSGTRTSESKIMTDIISIGSDNEMHENDESVTR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23276153)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EQTN Antibody

Western Blot: EQTN Antibody [NBP1-90869]

Western Blot: EQTN Antibody [NBP1-90869]

Western Blot: C9orf11 Antibody [NBP1-90869] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]

Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]

Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]

Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]

Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90869 (A) shows similar protein distribution across tissues to independent antibody NBP1-90868 (B).

Applications for EQTN Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EQTN

Alternate Names

acrosome formation associated factor, acrosome formation-associated factor, AFAF, C9orf11, chromosome 9 open reading frame 11, equatorin, equatorin, sperm acrosome associated, SPACA8

Gene Symbol

EQTN

Additional EQTN Products

Product Documents for EQTN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EQTN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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