EQTN Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90869
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SCESQYSVNPELATMSYFHPSEGVSDTSFSKSAESSTFLGTTSSDMRRSGTRTSESKIMTDIISIGSDNEMHENDESVTR
Reactivity Notes
Reactivity reported in scientific literature (PMID: 23276153)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EQTN Antibody
Western Blot: EQTN Antibody [NBP1-90869]
Western Blot: C9orf11 Antibody [NBP1-90869] - Analysis in control (vector only transfected HEK293T lysate) and EQTN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon shows no positivity in glandular cells as expected.Immunohistochemistry-Paraffin: EQTN Antibody [NBP1-90869]
Immunohistochemistry-Paraffin: C9orf11 Antibody [NBP1-90869] - Staining of human colon, liver, prostate and testis using Anti-EQTN antibody NBP1-90869 (A) shows similar protein distribution across tissues to independent antibody NBP1-90868 (B).Applications for EQTN Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EQTN
Alternate Names
acrosome formation associated factor, acrosome formation-associated factor, AFAF, C9orf11, chromosome 9 open reading frame 11, equatorin, equatorin, sperm acrosome associated, SPACA8
Gene Symbol
EQTN
Additional EQTN Products
Product Documents for EQTN Antibody
Product Specific Notices for EQTN Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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