ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33321

Novus Biologicals, part of Bio-Techne
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NBP2-33321-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ER506

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Summary for ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

Immunogen

Recombinant fragment (around aa 2-185) of human ER alpha/NR3A1 protein (exact sequence is proprietary) (Uniprot: P03372)

Localization

Nucleus

Specificity

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Marker

Marker of Estrogen Dependence

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-33320).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

Western Blot: ER alpha/NR3A1 Antibody (ER506)Azide and BSA Free [NBP2-33321]

Western Blot: ER alpha/NR3A1 Antibody (ER506)Azide and BSA Free [NBP2-33321]

Western Blot: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321]

Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).
Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321]

Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321]

Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 Antibody (ER506).
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Applications for ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

The estrogen receptor (ER) is a ~ 67kDa protein, originally described by Jensen and Jacobson, 1962 as a uterine protein which bound 3H-estradiol with high affinity. More than two decades later the cDNA encoding the human estrogen receptor was finally cloned and sequenced. The estrogen receptor gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. The A/B domain of the estrogen receptor contains a constitutively active transcription activation function called TAF-1. Domain C contains two Cys4 zinc fingers, which form the core of the DNA binding domain; it also contains a weak constitutive dimerization activity. The zinc fingers interact with DNA, and the ER homodimer appears to bind most tightly to the palindromic estrogen response element (ERE) sequence GGATCNNNGATCC. The hinge domain D appears to be the location for binding by heat shock proteins. The estrogen receptor is an important regulator of growth and differentiation in the mammary gland. Estrogens play an important role also in breast cancer development, although tumors often progress towards an autocrine growth. This hormone-independence and the incomplete correlation between ER-status and response to endocrine (tamoxifen) therapy may be explained by the presence of receptor variants with either constitutive transcriptional activity or a dominant negative inhibition of normal ER function. Many investigators have reported on the isolation of cDNAs which encode variant ER sequences. Many of these variants appear to be formed by errors in RNA splicing which result in one or more exons being lost during processing of the ER message.

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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