Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D3

Concentration

1 mg/ml

View all ER alpha/NR3A1 Antibodies »

Product Summary for ER alpha/NR3A1 Antibody (OTI2D3)

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-310 of human ESR1 (NP_000116) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ER alpha/NR3A1 Antibody (OTI2D3)

Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Western Blot: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Western Blot: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ER alpha/NR3A1.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI2D3) [NBP2-45522]

Immunohistochemistry: Estrogen Receptor Antibody (2D3) [NBP2-45522] - Analysis of Human breast tissue.
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Applications for ER alpha/NR3A1 Antibody (OTI2D3)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3).

Long Name

Estrogen Receptor alpha

Alternate Names

ESR1, NR3A1

Gene Symbol

ESR1

Additional ER alpha/NR3A1 Products

Product Documents for ER alpha/NR3A1 Antibody (OTI2D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ER alpha/NR3A1 Antibody (OTI2D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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