ER alpha/NR3A1 Antibody (rESR1/8761)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20618
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2b Kappa Clone # rESR1/8761
Concentration
0.2 mg/ml
Product Summary for ER alpha/NR3A1 Antibody (rESR1/8761)
Immunogen
Recombinant fragment corresponding to C-terminus of human ER alpha/NR3A1
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Positive Controls: Human breast or endometrial carcinoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for ER alpha/NR3A1 Antibody (rESR1/8761)
ER alpha/NR3A1 Antibody (rESR1/8761)
Formalin fixed paraffin embedded human breast carcinoma stained with ER alpha/NR3A1 antibody (rESR1/8761). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for ER alpha/NR3A1 Antibody (rESR1/8761)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ER alpha/NR3A1
Long Name
Estrogen Receptor alpha
Alternate Names
ESR1, NR3A1
Gene Symbol
ESR1
Additional ER alpha/NR3A1 Products
Product Documents for ER alpha/NR3A1 Antibody (rESR1/8761)
Product Specific Notices for ER alpha/NR3A1 Antibody (rESR1/8761)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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