ErbB2/Her2 Antibody - BSA Free

Western Blot: ErbB2/Her2 AntibodyBSA Free [NBP2-29624]

Western Blot: ErbB2/HER2 Antibody [NBP2-29624] - Western blot analysis of ErbB2/HER2 in SKOV3 cell lysate.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB3 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. The cells in the cores of some tumor areas were negative for ErbB2.

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody - BSA Free [NBP2-29624]

Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP2-29624] - IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:50 dilution of ErbB2/HER2 antibody (NBP2-29624) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This ErbB2 antibody generated a diffused cytoplasmic with some membrane and nuclear staining in the cancer cells, endothelial cells as well as in the tumor stromal cells. Staining was performed by Histowiz.