ErbB4/Her4 Antibody (JE59-43)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32314
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE59-43
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human ErbB4/Her4 aa 1,231-1,280 / 1,308. (Uniprot: Q15303)
Localization
Cell membrane; Nucleus, Mitochondrion.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
147 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ErbB4/Her4 Antibody (JE59-43)
Western Blot: ErbB4/Her4 Antibody (JE59-43) [NBP3-32314]
Western Blot: ErbB4/Her4 Antibody (JE59-43) [NBP3-32314] - Western blot analysis of ErbB4/Her4 on different lysates with Rabbit anti-ErbB4/Her4 antibody (NBP3-32314) at 1/1,000 dilution.Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 ug/Lane.
Predicted band size: 147 kDa
Observed band size: 180 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32314) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
Applications for ErbB4/Her4 Antibody (JE59-43)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ErbB4/Her4
Long Name
Receptor Tyrosine Protein Kinase ErbB4
Alternate Names
HER4
Gene Symbol
ERBB4
Additional ErbB4/Her4 Products
Product Documents for ErbB4/Her4 Antibody (JE59-43)
Product Specific Notices for ErbB4/Her4 Antibody (JE59-43)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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