ERCC1 Antibody (OTI1A3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70662

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ERCC1 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human ERCC1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERCC1 Antibody (OTI1A3) - Azide and BSA Free

Western Blot: ERCC1 Antibody (OTI1A3)Azide and BSA Free [NBP2-70662]

Western Blot: ERCC1 Antibody (OTI1A3)Azide and BSA Free [NBP2-70662]

Western Blot: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662]

Immunocytochemistry/ Immunofluorescence: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662]

Immunocytochemistry/Immunofluorescence: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1.
Immunohistochemistry: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662]

Immunohistochemistry: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662]

Immunohistochemistry: ERCC1 Antibody (OTI1A3) - Azide and BSA Free [NBP2-70662] - Staining of paraffin-embedded prostate tissue using anti-ERCC1 mouse monoclonal antibody.
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Applications for ERCC1 Antibody (OTI1A3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERCC1

The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1 XPF complex makes the 5'incision. Elevated levels of ERCC1 have also been reported in Cisplatin resistant cells.

Long Name

Excision Repair Cross-complementing 1

Alternate Names

COFS4, DNA excision repair protein ERCC-1, excision repair cross-complementing rodent repair deficiency, complementationgroup 1 (includes overlapping antisense sequence), RAD10, UV20

Gene Symbol

ERCC1

Additional ERCC1 Products

Product Documents for ERCC1 Antibody (OTI1A3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERCC1 Antibody (OTI1A3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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