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ERCC6L Antibody (3F12-2B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00054821-M01

Novus Biologicals, part of Bio-Techne
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H00054821-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F12-2B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ERCC6L (AAH08808, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN

Specificity

FLJ20105 - FLJ20105 protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ERCC6L Antibody (3F12-2B10)

Western Blot: ERCC6L Antibody (3F12-2B10) [H00054821-M01]

Western Blot: ERCC6L Antibody (3F12-2B10) [H00054821-M01]

Western Blot: ERCC6L Antibody (3F12-2B10) [H00054821-M01] - Analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody (M01), clone 3F12-2B10.Lane 1: ERCC6L transfected lysate(46.2 KDa).Lane 2: Non-transfected lysate.
ELISA: ERCC6L Antibody (3F12-2B10) [H00054821-M01]

ELISA: ERCC6L Antibody (3F12-2B10) [H00054821-M01]

ELISA: ERCC6L Antibody (3F12-2B10) [H00054821-M01] - Detection limit for recombinant GST tagged ERCC6L is approximately 0.03ng/ml as a capture antibody.

Applications for ERCC6L Antibody (3F12-2B10)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 28904335).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERCC6L

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase

Alternate Names

ATP-dependent helicase ERCC6-like, DNA excision repair protein ERCC-6-like, EC 3.6.1, EC 3.6.4.12, excision repair cross-complementing rodent repair deficiency, complementationgroup 6-like, excision repair protein ERCC6-like, FLJ20105, MGC131695, PICHexcision repair cross-complementing rodent repair deficiency complementationgroup 6 - like, PLK1-interacting checkpoint helicase, SNF2/RAD54 family protein, Tumor antigen BJ-HCC-15

Entrez Gene IDs

54821 (Human)

Gene Symbol

ERCC6L

Additional ERCC6L Products

Product Documents for ERCC6L Antibody (3F12-2B10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERCC6L Antibody (3F12-2B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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