ERF Antibody (3F11)

Novus Biologicals, part of Bio-Techne | Catalog # H00002077-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3F11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERF Antibodies »

Product Specifications

Immunogen

ERF (AAH22231, 181 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LGRGSVSDCSDGTSELEEPLGEDPRARPPGPPDLGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPSPTLSPMYPSG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

ERF - Ets2 repressor factor (3F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ERF Antibody (3F11)

Western Blot: ERF Antibody (3F11) [H00002077-M02]

Western Blot: ERF Antibody (3F11) [H00002077-M02]

Western Blot: ERF Antibody (3F11) [H00002077-M02] - ERF monoclonal antibody (M02), clone 3F11. Analysis of ERF expression in Raw 264.7.
Immunocytochemistry/ Immunofluorescence: ERF Antibody (3F11) [H00002077-M02]

Immunocytochemistry/ Immunofluorescence: ERF Antibody (3F11) [H00002077-M02]

Immunocytochemistry/Immunofluorescence: ERF Antibody (3F11) [H00002077-M02] - Analysis of monoclonal antibody to ERF on HeLa cell. Antibody concentration 10 ug/ml.

Applications for ERF Antibody (3F11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERF

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM]

Alternate Names

Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF

Entrez Gene IDs

2077 (Human)

Gene Symbol

ERF

UniProt

AAH22231 (Human)

Additional ERF Products

Product Documents for ERF Antibody (3F11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERF Antibody (3F11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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