Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ERF Antibodies »

Product Summary for ERF Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 498 and 548 of human Ets2 repressor factor using the numbering given in entry NP_006485.2 (GeneID 2077).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERF Antibody

Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120]

Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120]

Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB
Immunoprecipitation: ERF Antibody [NB100-88120]

Immunoprecipitation: ERF Antibody [NB100-88120]

Immunoprecipitation: ERF Antibody [NB100-88120] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88120 used for IP at 3 mcg/mg lysate. ERF was also immunoprecipitated by NB100-88119, which recognizes an upstream epitope.

Applications for ERF Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 - 5 ug/mg lysate
Application Notes
Immunohistochemistry-Paraffin: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Western Blot: This antibody has not performed satisfactorily when used for western blot of ERF in crude preparations (e.g. whole cell lysate). This antibody can be used for western blot of enriched (e.g. immunoprecipitated) sources of ERF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ERF

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM]

Alternate Names

Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF

Entrez Gene IDs

2077 (Human)

Gene Symbol

ERF

UniProt

NP_006485.2 (Human)

Additional ERF Products

Product Documents for ERF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ERF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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