ERF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16366

Novus Biologicals, part of Bio-Techne
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NBP2-16366
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ERF Antibodies »

Product Summary for ERF Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERF. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERF Antibody

Western Blot: ERF Antibody [NBP2-16366]

Western Blot: ERF Antibody [NBP2-16366]

Western Blot: ERF Antibody [NBP2-16366] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ERF Antibody [NBP2-16366]

Immunocytochemistry/ Immunofluorescence: ERF Antibody [NBP2-16366]

Immunocytochemistry/Immunofluorescence: ERF Antibody [NBP2-16366] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERF stained by ERF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.
Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366]

Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366]

Immunohistochemistry-Paraffin: ERF Antibody [NBP2-16366] - Mouse testis. ERF stained by ERF antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
View 2 more images

Applications for ERF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ERF

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM]

Alternate Names

Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF

Gene Symbol

ERF

UniProt

P50548 (Human)

Additional ERF Products

Product Documents for ERF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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