eRF1 Antibody (OTI2E3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70692

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all eRF1 Antibodies »

Product Summary for eRF1 Antibody (OTI2E3) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

48.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eRF1 Antibody (OTI2E3) - Azide and BSA Free

Western Blot: eRF1 Antibody (OTI2E3)Azide and BSA Free [NBP2-70692]

Western Blot: eRF1 Antibody (OTI2E3)Azide and BSA Free [NBP2-70692]

Western Blot: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRF1.
Flow Cytometry: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692]

Flow Cytometry: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692]

Flow Cytometry: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - Analysis of Jurkat cells, using anti-eRF1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: eRF1 Antibody (OTI2E3)Azide and BSA Free [NBP2-70692]

Western Blot: eRF1 Antibody (OTI2E3)Azide and BSA Free [NBP2-70692]

Western Blot: eRF1 Antibody (OTI2E3) - Azide and BSA Free [NBP2-70692] - Analysis of extracts (35ug) from 9 different cell lines by using anti-eRF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549:human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Applications for eRF1 Antibody (OTI2E3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eRF1

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Alternate Names

D5S1995, ERF1eRF1, eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eukaryotic translation termination factor 1, polypeptide chain release factor 1, Protein Cl1, RF1ERF, sup45 (yeast omnipotent suppressor 45) homolog-like 1, SUP45L1, TB3-1MGC111066

Gene Symbol

ETF1

Additional eRF1 Products

Product Documents for eRF1 Antibody (OTI2E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eRF1 Antibody (OTI2E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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