ERG Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60655
![Western Blot: ERG AntibodyBSA Free [NBP2-60655]](https://resources.bio-techne.com/images/products/ERG-Antibody-Western-Blot-NBP2-60655-img0002.jpg "Western Blot: ERG AntibodyBSA Free [NBP2-60655]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human ERG protein (amino acids 13-114) [UniPro P11308]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ERG Antibody - BSA Free
Western Blot: ERG AntibodyBSA Free [NBP2-60655]
Western Blot: ERG Antibody [NBP2-60655] - Total protein from Human THP-1, Jurkat and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ERG in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunocytochemistry/ Immunofluorescence: ERG Antibody - BSA Free [NBP2-60655]](https://resources.bio-techne.com/images/products/ERG-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60655-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ERG Antibody - BSA Free [NBP2-60655]")
Immunocytochemistry/ Immunofluorescence: ERG Antibody - BSA Free [NBP2-60655]
Immunocytochemistry/Immunofluorescence: ERG Antibody [NBP2-60655] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ERG at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: ERG Antibody - BSA Free [NBP2-60655]](https://resources.bio-techne.com/images/products/ERG-Antibody-Immunohistochemistry-Paraffin-NBP2-60655-img0003.jpg "Immunohistochemistry-Paraffin: ERG Antibody - BSA Free [NBP2-60655]")
Immunohistochemistry-Paraffin: ERG Antibody - BSA Free [NBP2-60655]
Immunohistochemistry-Paraffin: ERG Antibody [NBP2-60655] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney (NBP1-78269) using 1:100 dilution of ERG antibody. The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity in the cells of the glomeruli and the tubular epithelial cells.Applications for ERG Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500
Immunohistochemistry
1:100 - 1:200
Immunohistochemistry-Paraffin
1:100-1:200
Western Blot
1 - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Erg
Long Name
v-Ets Erythroblastosis Virus E26 Oncogene Homolog
Alternate Names
Erg-3, p55
Gene Symbol
ERG
Additional Erg Products
Product Documents for ERG Antibody - BSA Free
Product Specific Notices for ERG Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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