ERK5/BMK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42673

Novus Biologicals, part of Bio-Techne
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NBP1-42673
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Rat

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ERK5/BMK1 Antibodies »

Product Summary for ERK5/BMK1 Antibody

Immunogen

The immunogen for this product maps to a region between residue 766 and 816 of human extracellular-signal-regulated kinase 5 using the numbering given in entry NP_002740.2 (GeneID 5598).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERK5/BMK1 Antibody

Western Blot: ERK5/BMK1 Antibody [NBP1-42673]

Western Blot: ERK5/BMK1 Antibody [NBP1-42673]

Western Blot: ERK5/BMK1 Antibody [NBP1-42673] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ERK5 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ERK5 was also immunoprecipitated by rabbit anti-ERK5 antibody NBP1-42672, which recognizes an upstream epitope.

Applications for ERK5/BMK1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-42673 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ERK5/BMK1

The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5).

Long Name

Extracellular Signal-regulated Kinase 5

Alternate Names

BMK1, MAPK7

Entrez Gene IDs

5598 (Human)

Gene Symbol

MAPK7

Additional ERK5/BMK1 Products

Product Documents for ERK5/BMK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERK5/BMK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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