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ERP44 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97000

Novus Biologicals, part of Bio-Techne
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NBP2-97000-100ul
NBP2-97000-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ERP44 (Accession#: NP_055866.1; Met1-Asp402)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ERP44 Antibody

Western Blot: ERP44 Antibody [NBP2-97000]

Western Blot: ERP44 Antibody [NBP2-97000]

Western Blot: ERP44 Antibody [NBP2-97000] - Anti-ERP44 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lane B: NCI-H1299 Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa
Immunoprecipitation: ERP44 Antibody [NBP2-97000]

Immunoprecipitation: ERP44 Antibody [NBP2-97000]

Immunoprecipitation: ERP44 Antibody [NBP2-97000] - ERP44 was immunoprecipitated using: Lane A: 0.5 mg Raji Whole Cell Lysate. 2 ul anti-ERP44 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-ERP44 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa

Applications for ERP44 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERP44

ERP44 mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protei

Alternate Names

endoplasmic reticulum protein 44, endoplasmic reticulum resident protein 44 kDa, ER protein 44, ERp44, KIAA0573endoplasmic reticulum resident protein 44, PDIA10, protein disulfide isomerase family A, member 10, thioredoxin domain containing 4 (endoplasmic reticulum), Thioredoxin domain-containing protein 4, TXNDC4

Gene Symbol

ERP44

Additional ERP44 Products

Product Documents for ERP44 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ERP44 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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