Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Hamster, Monkey, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Map.ERp57 (GRP58)

Concentration

1 mg/ml

View all ERp57/PDIA3 Antibodies »

Product Summary for ERp57/PDIA3 Antibody (Map.ERp57 (GRP58))

Immunogen

Human recombinant ERp57 (Grp58)

Reactivity Notes

Guinea Pig (Cavia porcellus)

Localization

Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERp57/PDIA3 Antibody (Map.ERp57 (GRP58))

Western Blot: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Western Blot: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Western Blot: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689] - Analysis of Human cell lysates showing detection of Erp57 protein using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689] - Analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic and perinuclear staining.
Immunohistochemistry: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Immunohistochemistry: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689]

Immunohistochemistry: ERp57/PDIA3 Antibody (Map.ERp57 (GRP58)) [NBP2-59689] - Immunohistochemistry analysis using Mouse Anti-Erp57 Monoclonal Antibody, Clone Map.ERP57 (NBP2-59689). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Erp57 Monoclonal Antibody (NBP2-59689) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis and Hair Follicles.

Applications for ERp57/PDIA3 Antibody (Map.ERp57 (GRP58))

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of this antibody was sufficient for detection of ERp57 in 10 ug of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody (Map.ERp57 (GRP58))

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ERp57/PDIA3 Antibody (Map.ERp57 (GRP58))

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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