ERp72 Antibody (S02-1J3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19626

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19626-100ul
NBP3-19626-50ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-1J3

Concentration

0.3 mg/ml

View all ERp72 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human ERp72 (Uniprot # P13667)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERp72 Antibody (S02-1J3)

Western Blot: ERp72 Antibody (S02-1J3) [NBP3-19626]

Western Blot: ERp72 Antibody (S02-1J3) [NBP3-19626]

Western Blot: ERp72 Antibody (S02-1J3) [NBP3-19626] - Western blot detection of ERp72 in PC-3 cell lysates using NBP3-19626 (1:1000 diluted). Predicted band size: 73kDa. Observed band size: 73kDa.

Applications for ERp72 Antibody (S02-1J3)

Application
Recommended Usage

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp72

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Alternate Names

Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ER protein 70, ERp70, ERP70ER protein 72, ERp72, ERp-72, ERP72EC 5.3.4.1, intestinal-related), protein disulfide isomerase family A, member 4, protein disulfide isomerase related protein (calcium-binding protein, protein disulfide isomerase-associated 4, protein disulfide-isomerase A4

Gene Symbol

PDIA4

Additional ERp72 Products

Product Documents for ERp72 Antibody (S02-1J3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERp72 Antibody (S02-1J3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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