ERR alpha/NR3B1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47254

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ERR alpha/NR3B1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of human Estrogen Related Receptor alpha (within residues 1-50). [Swiss-Prot# P11474]

Reactivity Notes

Predicted to react with rat, pig and dog based on 100% sequence homology.

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ERR alpha/NR3B1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

Western Blot: ERR alpha/NR3B1 AntibodyBSA Free [NBP1-47254]

Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P <0.05), NS = non-significant (P>0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (https://nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody - BSA Free [NBP1-47254]

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)
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Applications for ERR alpha/NR3B1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

2 ug/ml
Application Notes
This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 3 using NBP1-47254 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERR alpha/NR3B1

Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.

Long Name

Estrogen-related Receptor alpha

Alternate Names

ERR1, ESRL1, ESRRA, NR3B1

Entrez Gene IDs

2101 (Human); 26379 (Mouse); 293701 (Rat)

Gene Symbol

ESRRA

UniProt

P11474 (Human)

Additional ERR alpha/NR3B1 Products

Product Documents for ERR alpha/NR3B1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERR alpha/NR3B1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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