ERR beta/NR3B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-748

Novus Biologicals, part of Bio-Techne
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NB300-748
NB300-748-0.025mg
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Key Product Details

Species Reactivity

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all ERR beta/NR3B2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2.

Specificity

Estrogen-Related Receptor beta

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ERR beta/NR3B2 Antibody

Western Blot: ERR beta/NR3B2 Antibody [NB300-748]

Western Blot: ERR beta/NR3B2 Antibody [NB300-748]

Western Blot: ERR beta/NR3B2 Antibody [NB300-748] - Analysis of ERR beta in HeLa whole cell lysate. Image courtesy of anonymous customer.
Immunocytochemistry/ Immunofluorescence: ERR beta/NR3B2 Antibody [NB300-748]

Immunocytochemistry/ Immunofluorescence: ERR beta/NR3B2 Antibody [NB300-748]

Immunocytochemistry/Immunofluorescence: ERR beta/NR3B2 Antibody [NB300-748] - Analysis of Estrogen Related Receptor beta (green) in NRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a Estrogen Related Receptor beta polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Western Blot: ERR beta/NR3B2 Antibody [NB300-748]

Western Blot: ERR beta/NR3B2 Antibody [NB300-748]

Western Blot: ERR beta/NR3B2 Antibody [NB300-748] - Analysis of rat kidney.

Applications for ERR beta/NR3B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB300-748 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERR beta/NR3B2

Estrogen related receptor beta, a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta.

Long Name

Estrogen-related Receptor beta

Alternate Names

ERR2, ESRL2, ESRRB, NR3B2

Gene Symbol

ESRRB

Additional ERR beta/NR3B2 Products

Product Documents for ERR beta/NR3B2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERR beta/NR3B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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