ERR gamma/NR3B3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-00182

Novus Biologicals, part of Bio-Techne
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NBP1-00182
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ERR gamma/NR3B3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRMSNKDRHIDS corresponding to internal region according to NP_001429.2, NP_996317.1, NP_996318.1.

Specificity

This antibody is expected to recognise the reported isoform NP_001429.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ERR gamma/NR3B3 Antibody

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182] - HEK293 overexpressing ESRRG and probed with (mock transfection in first lane).
Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182]

Western Blot: ERR gamma/NR3B3 Antibody [NBP1-00182] - (0.2ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ERR gamma/NR3B3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human brain (cerebellum) and breast lysates (calculated MW of 51.3 kDa band according to NP_NP_001429.2). In transfected HEK293 transiently expressing ESRRG a band of approx. 52 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERR gamma/NR3B3

Estrogen related receptor (ERR gamma), a NR3 Steroid Receptor is an orphan member of the nuclear hormone receptor superfamily. All the ERR family members (1,2, and 3) share an almost identical DNA binding domain, which has 68% amino acid identity with that of estrogen receptor. Studies involving the crystal structure of the LBD have shown a complex between ERR3 and SRC1. ERR gamma has been suggested to affect differentiation of the brain, heart, and kidney. ERR gamma binds as a monomer to an extended half site of the ERRE type (TCAAGGTCA). ERR gamma has been shown to interact with PGC1 alpha and has been implicated in the regulation of mitochondrial energy metabolism. In humans, ERR gamma pre mRNA undergoes extensive alternative splicing at the 5 prime end, yielding at least six mRNA splice variants and two protein isoforms that differ by 23 amino acids in the N terminus. ERR gamma has been shown to be overexpressed in breast tumors, and its expression is correlated with levels of ErbB4, a likely indicator of preferred clinical course. As a result, ERR gamma has been suggested to be a potential biomarker for favorable clinical course and, possibly, hormonal sensitivity, and as a candidate target for therapeutic development.

Long Name

Estrogen-related Receptor gamma

Alternate Names

ERR3, ESRRG, NR3B3

Entrez Gene IDs

2104 (Human); 26381 (Mouse)

Gene Symbol

ESRRG

UniProt

NP_001429.2 (Human)

Additional ERR gamma/NR3B3 Products

Product Documents for ERR gamma/NR3B3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ERR gamma/NR3B3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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