espin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90588

Novus Biologicals, part of Bio-Techne
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NBP1-90588
NBP1-90588-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all espin Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPWQRQVILKK

Reactivity Notes

Rat reactivity reported in (PMID:30467855). Mouse (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for espin Antibody

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining in human testis and skeletal muscle tissues using NBP1-90588 antibody. Corresponding ESPN RNA-seq data are presented for the same tissues.
Western Blot: espin Antibody [NBP1-90588]

Western Blot: espin Antibody [NBP1-90588]

Western Blot: espin Antibody [NBP1-90588] - Analysis in human cell line SK-BR-3.
Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588]

Immunohistochemistry-Paraffin: espin Antibody [NBP1-90588] - Staining of human duodenum shows strong positivity in apical membrane in glandular cells.
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Applications for espin Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:20

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: espin

Alternate Names

autosomal recessive deafness type 36 protein, DKFZp434A196, DKFZp434G2126, espin

Entrez Gene IDs

83715 (Human)

Gene Symbol

ESPN

UniProt

B1AK53 (Human)

Additional espin Products

Product Documents for espin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for espin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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