ESRP2 Antibody (22C5.H7.A6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77972
![Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972]](https://resources.bio-techne.com/images/products/ESRP2-Antibody-22C5-H7-A6-Western-Blot-NBP1-77972-img0002.jpg "Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 22C5.H7.A6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ESRP2 Antibody (22C5.H7.A6) is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein. (Uniprot: Q8K0G8)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with ESRP2 from mouse based on a 100% homology with the immunizing sequence. Cross-reactivity with ESRP2 from other sources has not been determined.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
This antibody is an IgG fraction antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ESRP2 Antibody (22C5.H7.A6)
Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972]
Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972] - Anti-ESRP2 by Detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2.Applications for ESRP2 Antibody (22C5.H7.A6)
Application
Recommended Usage
ELISA
1:100000
Western Blot
1:1000
Application Notes
This product has been tested for use western blotting and ELSIA. This antibody shows specific reactivity by with ESRP2 transfected cell lysates. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract. The antibody shows bands at ~77kD and 70kD and no reactivity is observed in cells transfected with GFP or ESRP1. A starting dilution of 1:1000 is suggested for western blotting.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: ESRP2
Alternate Names
epithelial splicing regulatory protein 2, FLJ21918, FLJ22248, RBM35BRNA-binding protein 35B, RNA binding motif protein 35A, RNA binding motif protein 35B, RNA-binding motif protein 35B
Gene Symbol
ESRP2
UniProt
Additional ESRP2 Products
Product Documents for ESRP2 Antibody (22C5.H7.A6)
Product Specific Notices for ESRP2 Antibody (22C5.H7.A6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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