Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ESRP2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B. The immunogen is located within the last 50 amino acids of RBM35B. Amino Acid Squence: PADDYTSLMPVGDPPRT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Specificity

RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ESRP2 Antibody - BSA Free

Western Blot: ESRP2 AntibodyBSA Free [NBP1-76537]

Western Blot: ESRP2 AntibodyBSA Free [NBP1-76537]

Western Blot: ESRP2 Antibody [NBP1-76537] - Human lung tissue lysate with ESRP2 antibody at 1 ug/mL.
ESRP2 Antibody - BSA Free

Immunohistochemistry: ESRP2 Antibody - BSA Free [NBP1-76537] -

Immunohistochemistry: ESRP2 Antibody - BSA Free [NBP1-76537] - Immunohistochemistry of ESRP2 in human lung tissue with ESRP2 antibody at 5 ug/mL.
ESRP2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ESRP2 Antibody - BSA Free [NBP1-76537] -

Immunocytochemistry/ Immunofluorescence: ESRP2 Antibody - BSA Free [NBP1-76537] - Immunofluorescence of ESRP2 in human lung tissue cells with ESRP2 antibody at 20 ug/mL.

Applications for ESRP2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ESRP2

RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.

Alternate Names

epithelial splicing regulatory protein 2, FLJ21918, FLJ22248, RBM35BRNA-binding protein 35B, RNA binding motif protein 35A, RNA binding motif protein 35B, RNA-binding motif protein 35B

Gene Symbol

ESRP2

UniProt

NP_079215 (Human)

Additional ESRP2 Products

Product Documents for ESRP2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ESRP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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