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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # ETS1/1801

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Ets-1 Antibody (ETS1/1801) - Azide and BSA Free

Immunogen

Recombinant human Ets-1 fragment (around aa137-230) (exact sequence is proprietary) (Uniprot: P14921)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29858063).

Localization

Nuclear

Marker

Marker of Tumor Metastasis

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53385).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Ets-1 Antibody (ETS1/1801) - Azide and BSA Free

Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514]

Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514]

Protein Array: Ets-1 Antibody (ETS1/1801) - Azide and BSA Free [NBP2-54514] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for Ets-1 Antibody (ETS1/1801) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Ets-1

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1

Long Name

E26 Transformation-specific Sequence-1

Alternate Names

Ets1, EWSR2, p54, Protein C-Ets-1, v-ets

Gene Symbol

ETS1

Additional Ets-1 Products

Product Documents for Ets-1 Antibody (ETS1/1801) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ets-1 Antibody (ETS1/1801) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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