ETS2 Antibody (PCRP-ETS2-1D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13877

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-ETS2-1D9

Concentration

0.2 mg/ml

View all ETS2 Antibodies »

Product Summary for ETS2 Antibody (PCRP-ETS2-1D9)

Immunogen

Recombinant full-length human ETS2 protein (Uniprot: P15036)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14209)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for ETS2 Antibody (PCRP-ETS2-1D9)

Immunocytochemistry/ Immunofluorescence: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Immunocytochemistry/ Immunofluorescence: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Immunocytochemistry/Immunofluorescence: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using ETS2 Antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Flow Cytometry: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Flow Cytometry: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Flow cytometric analysis of PFA-fixed HeLa cells. ETS2 antibody (PCRP-ETS2-1D9) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Protein Array: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877]

Protein Array: ETS2 Antibody (PCRP-ETS2-1D9) [NBP3-13877] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ETS2 Antibody (PCRP-ETS2-1D9).

Applications for ETS2 Antibody (PCRP-ETS2-1D9)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ETS2

ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (Dwyer et al., 2007 [PubMed 17986575]).[supplied by OMIM]

Alternate Names

ETS2IT1, human erythroblastosis virus oncogene homolog 210protein C-ets-2, oncogene ETS-2, v-ets avian erythroblastosis virus E2 oncogene homolog 2, v-ets avian erythroblastosis virus E26 oncogene homolog 2, v-ets erythroblastosis virus E26 oncogene homolog 2 (avian)

Gene Symbol

ETS2

UniProt

P15036 (Human)

Additional ETS2 Products

Product Documents for ETS2 Antibody (PCRP-ETS2-1D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ETS2 Antibody (PCRP-ETS2-1D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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