Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71554
![Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71554-img0003.jpg "Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]")
![Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9)Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Western-Blot-NBP2-71554-img0001.jpg "Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9)Azide and BSA Free [NBP2-71554]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2B9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human EIF1 (NP_005792) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9)Azide and BSA Free [NBP2-71554]
Western Blot: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Eukaryotic translation initiation factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and imm![Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71554-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]")
Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]
Immunocytochemistry/Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Eukaryotic translation initiation factor 1.![Immunohistochemistry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71554-img0007.jpg "Immunohistochemistry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]")
Immunohistochemistry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]
Immunohistochemistry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554] - Staining of paraffin-embedded Human lymph node tissue using anti-Eukaryotic translation initiation factor 1 mouse monoclonal antibody.![Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71554-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]")
![Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]](https://resources.bio-techne.com/images/products/Eukaryotic-translation-initiation-factor-1-Antibody-OTI2B9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71554-img0002.jpg "Flow Cytometry: Eukaryotic translation initiation factor 1 Antibody (OTI2B9) - Azide and BSA Free [NBP2-71554]")
Applications
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Eukaryotic translation initiation factor 1
Alternate Names
A121Protein translation factor SUI1 homolog, eIF1, EIF-1, EIF1A, eukaryotic translation initiation factor 1, ISO1, Sui1iso1, SUI1sui1iso1
Gene Symbol
EIF1
Additional Eukaryotic translation initiation factor 1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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