EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71553

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all EVA1 Antibodies »

Product Summary for EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human MPZL2(NP_658911) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

Western Blot: EVA1/MPZL2 Antibody (OTI2C7)Azide and BSA Free [NBP2-71553]

Western Blot: EVA1/MPZL2 Antibody (OTI2C7)Azide and BSA Free [NBP2-71553]

Western Blot: EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free [NBP2-71553] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPZL2.
Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free [NBP2-71553]

Immunocytochemistry/ Immunofluorescence: EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free [NBP2-71553]

Immunocytochemistry/Immunofluorescence: EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free [NBP2-71553] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MPZL2.

Applications for EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EVA1

Thymus development depends on a complex series of interactions between thymocytes and the stromal component of the organ. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and strongly downregulated by thymocyte developmental progression. This gene is expressed in the thymus and in several epithelial structures early in embryogenesis. It is highly homologous to the myelin protein zero and, in thymus-derived epithelial cell lines, is poorly soluble in nonionic detergents, strongly suggesting an association to the cytoskeleton. Its capacity to mediate cell adhesion through a homophilic interaction and its selective regulation by T cell maturation might imply the participation of EVA in the earliest phases of thymus organogenesis. The protein bears a characteristic V-type domain and two potential N-glycosylation sites in the extracellular domain; a putative serine phosphorylation site for casein kinase 2 is also present in the cytoplasmic tail. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Long Name

Epithelial V-Like Antigen 1

Alternate Names

MPZL2

Gene Symbol

MPZL2

Additional EVA1 Products

Product Documents for EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EVA1/MPZL2 Antibody (OTI2C7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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