EVI-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52024

Novus Biologicals, part of Bio-Techne
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NBP1-52024
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all EVI-1 Antibodies »

Product Summary for EVI-1 Antibody

Immunogen

Peptide with sequence C-DKESLHSTSH corresponding to internal region according to NP_001098547.3, NP_005232.2, NP_004982.2, NP_001157471.1, NP_001157472.1.

Epitope

C-DKESLHSTSH

Specificity

This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EVI-1 Antibody

Western Blot: EVI-1 Antibody [NBP1-52024]

Western Blot: EVI-1 Antibody [NBP1-52024]

Western Blot: EVI-1 Antibody [NBP1-52024] - (2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EVI-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in A549 and HepG2 lysates (calculated MW of 126 kDa band according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EVI-1

EVI1 (ecotropic viral integration site 1) was originally identified as a gene located in the integration site of ecotropic retroviruses in the mouse genome that resulted in myeloid tumors. EVI1 is a zinc finger transcription factor that plays an important role in development and leukemogenesis. It is an oncogene that can have effects on cell proliferation, differentiation, and apoptosis. Chromosomal translocations involving EVI1 result in chronic myelogenous leukemia (CML). Alternate names for EVI1 include AML1-EVI-1 fusion protein, PRDM3, and MDS1-EV1.

Long Name

Ecotropic Viral Integration Site 1

Alternate Names

EVI1, MDS1, MECOM, PRDM3

Entrez Gene IDs

2122 (Human); 14013 (Mouse)

Gene Symbol

MECOM

UniProt

NP_001098547.3 (Human)

Additional EVI-1 Products

Product Documents for EVI-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EVI-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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