EWSR1 Antibody (9G3H1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16845

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16845-100ul
NBP3-16845-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9G3H1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EWSR1 Antibodies »

Product Summary for EWSR1 Antibody (9G3H1)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human EWSR1 (Q01844). MASTDYSTYSQAAAQQGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPTDVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTAPQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQSAYGTQPAYPAYGQQPAATAPTRPQDGNKPTETSQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQPVTAPPS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EWSR1 Antibody (9G3H1)

Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845]

Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845]

Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845]

Immunocytochemistry/ Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845]

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of U-2 OS cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845]

Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845]

Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry of paraffin-embedded mouse testis using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for EWSR1 Antibody (9G3H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EWSR1

The EWSR1 gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal

Long Name

RNA-binding protein EWS

Alternate Names

EWS, EWS oncogene

Gene Symbol

EWSR1

Additional EWSR1 Products

Product Documents for EWSR1 Antibody (9G3H1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EWSR1 Antibody (9G3H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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