Exendin-4 Antibody (35) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05179
![ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179]](https://resources.bio-techne.com/images/products/Exendin-4-Antibody-35-BSA-Free-ELISA-NBP1-05179-img0001.jpg "ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Reptile
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 35
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic exendin-4
Epitope
The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exedin (9-39)amide
Reactivity Notes
Reacts with Gila monster Heloderma suspectum
Specificity
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Exendin-4 Antibody (35) - BSA Free
ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179]
ELISA: Exendin-4 Antibody (35) - BSA Free [NBP1-05179] - Analysis using Biotin conjugate of NBP1-05179. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the NBP2-23578 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated NBP1-05179 as the detection antibody.Applications for Exendin-4 Antibody (35) - BSA Free
Application
Recommended Usage
ELISA
1:2000
Application Notes
ELISA: NBP1-05179 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between NBP1-05179 and exendin-4 is disrupted by 4.5 M MgCl2. NBP1-05179 (as biotinylated detection antibody) forms a sandwich ELISA pair with NBP1-05180 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 40 pmol/L in an unoptimized buffer assay. (1)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Exendin-4
Alternate Names
Exendin4
Additional Exendin-4 Products
Product Documents for Exendin-4 Antibody (35) - BSA Free
Product Specific Notices for Exendin-4 Antibody (35) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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