EXOC2 Antibody (JE65-98)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32322
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE65-98
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within Human EXOC2 aa 1-100 / 924. (Uniprot: Q96KP1)
Localization
Midbody, Midbody ring.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for EXOC2 Antibody (JE65-98)
Western Blot: EXOC2 Antibody (JE65-98) [NBP3-32322]
Western Blot: EXOC2 Antibody (JE65-98) [NBP3-32322] - Western blot analysis of EXOC2 on different lysates with Rabbit anti-EXOC2 antibody (NBP3-32322) at 1/1,000 dilution.Lane 1: K-562 cell lysate (15 ug/Lane)
Lane 2: Jurkat cell lysate (15 ug/Lane)
Lane 3: HepG2 cell lysate (15 ug/Lane)
Lane 4: MCF7 cell lysate (15 ug/Lane)
Lane 5: MDA-MB-231 cell lysate (15 ug/Lane)
Lane 6: HeLa cell lysate (15 ug/Lane)
Lane 7: HEK-293 cell lysate (15 ug/Lane)
Lane 8: U-2 OS cell lysate (15 ug/Lane)
Lane 9: Human brain tissue lysate (30 ug/Lane)
Lane 10: Mouse brain tissue lysate (30 ug/Lane)
Lane 11: Rat brain tissue lysate (30 ug/Lane)
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32322) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for EXOC2 Antibody (JE65-98)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EXOC2
Alternate Names
exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, SEC5-like 1, SEC5-like 1 (S. cerevisiae), Sec5p
Gene Symbol
EXOC2
Additional EXOC2 Products
Product Documents for EXOC2 Antibody (JE65-98)
Product Specific Notices for EXOC2 Antibody (JE65-98)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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