Exosome Detection (Western Blot) Antibody Pack
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11740
Key Product Details
Species
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Product Summary for Exosome Detection (Western Blot) Antibody Pack
This pack contains 1 vial each of: NBP2-77452SS (0.025 mg); MAB1663-SP (25 ug); MAB25292-SP (25 ug); NB100-65805SS (0.025 ml); NBP2-42225SS (0.025 ml); NBP1-47546SS (0.025 ml); MAB1455-SP (25 ug), HAF007 and HAF008.
Product Specifications
Specificity
Please see each vial's data sheet for specificity information specific to each product.
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for Exosome Detection (Western Blot) Antibody Pack
Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]
Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa) using CD81 Antibody (NB100-65805), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep36502), licensed under a CC-BY license.Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740]
Western Blot: Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysates of TIME human endothelial cell line and human platelets. PVDF membrane was probed with 2 ug/mL of Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB25292) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD9 at approximately 24 kDa (as indicated).
Exosome Detection (Western Blot) Antibody Pack [NBP3-11740] - Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 ug/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated).
Kit Contents for Exosome Detection (Western Blot) Antibody Pack
- MAB1455-SP
- MAB1663-SP
- MAB25292-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NB100-65805SS
- NBP1-47546SS
- NBP2-42225SS
- NBP2-77452SS
- HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Formulation, Preparation, and Storage
Formulation
See individual datasheets
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage is content dependent.
Background: Exosome Detection (Western Blot)
Alternate Names
EV detection, Exosome Markers, extracellular vesicle detection
Additional Exosome Detection (Western Blot) Products
Product Documents for Exosome Detection (Western Blot) Antibody Pack
Product Specific Notices for Exosome Detection (Western Blot) Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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