EYA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46136

Novus Biologicals, part of Bio-Techne
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NBP1-46136
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all EYA1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TDPTAEYSTIHSP corresponding to internal region according to NP_000494.2, NP_742056.1.

Specificity

This antibody is expected to recognize all isoforms (NP_742057.1; NP_000494.2; NP_742056.1). Reported variants represent identical protein: NP_000494.2, NP_742055.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

64.593 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EYA1 Antibody

Western Blot: EYA1 Antibody [NBP1-46136]

Western Blot: EYA1 Antibody [NBP1-46136]

Western Blot: EYA1 Antibody [NBP1-46136] - (2ug/ml) Analysis of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EYA1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in lysates of cell lline HEK293 (calculated MW of 60.7 kDa band according to NP_742056.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EYA1

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene.

Alternate Names

BOP, BOR, EC 3.1.3.48, eyes absent (Drosophila) homolog 1, eyes absent homolog 1, eyes absent homolog 1 (Drosophila), MGC141875

Entrez Gene IDs

2138 (Human); 14048 (Mouse); 502935 (Rat)

Gene Symbol

EYA1

UniProt

Q99502 (Human)

Additional EYA1 Products

Product Documents for EYA1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EYA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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