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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human eyes absent homolog 2 using the numbering given in entry NP_005235.3 (GeneID 2139).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA2 Antibody

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46816]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46816]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-46816] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EYA2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: EYA2 Antibody [NBP1-46816]

Immunoprecipitation: EYA2 Antibody [NBP1-46816]

Immunoprecipitation: EYA2 Antibody [NBP1-46816] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EYA2 antibody NBP1-46816 used for IP at 3 ug/mg lysate. EYA2 was also immunoprecipitated by rabbit anti-EYA2 antibody NBP1-46817, which recognizes a downstream epitope. For blotting immunoprecipitated EYA2, NBP1-46817 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for EYA2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Western Blot Not recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EYA2

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Five transcript variants encoding three distinct isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

EAB1EC 3.1.3.48, eyes absent (Drosophila) homolog 2, eyes absent homolog 2, eyes absent homolog 2 (Drosophila), MGC10614

Gene Symbol

EYA2

Additional EYA2 Products

Product Documents for EYA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EYA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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