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EYA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84026

Novus Biologicals, part of Bio-Techne
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NBP1-84026
NBP1-84026-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GTTGFYQGGNGLGNAAGFGSVHQDYPSYPGFPQSQYPQYYGSSYNPPYVPASSICPSPLSTSTYVLQEASHNVPNQSSESLAGEYNTHNGPSTPAKEGDTDRPHRASDGKLRGRSKRSSDPSPAGDNEIERVFVWDLD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (88%). Human reactivity reported in scientific literature (PMID: 24960160).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA2 Antibody

Western Blot: EYA2 Antibody [NBP1-84026]

Western Blot: EYA2 Antibody [NBP1-84026]

Western Blot: EYA2 Antibody [NBP1-84026] - Analysis in human cell line SCLC-21H.
Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-84026]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-84026]

Immunohistochemistry-Paraffin: EYA2 Antibody [NBP1-84026] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Western Blot: EYA2 Antibody [NBP1-84026]

Western Blot: EYA2 Antibody [NBP1-84026]

Western Blot: EYA2 Antibody [NBP1-84026] - Analysis in human cell line SCLC-21H.

Applications for EYA2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EYA2

EYA2 encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Five transcript variants encoding three distinct isoforms have been identified for this gene.

Alternate Names

EAB1EC 3.1.3.48, eyes absent (Drosophila) homolog 2, eyes absent homolog 2, eyes absent homolog 2 (Drosophila), MGC10614

Gene Symbol

EYA2

Additional EYA2 Products

Product Documents for EYA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EYA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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