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EYA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41100

Novus Biologicals, part of Bio-Techne
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NBP1-41100

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Summary for EYA4 Antibody

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human eyes absent homolog 4 using the numbering given in entry NP_004091.3 (GeneID 2070).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EYA4 Antibody

Western Blot: EYA4 Antibody [NBP1-41100]

Western Blot: EYA4 Antibody [NBP1-41100]

Western Blot: EYA4 Antibody [NBP1-41100] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EYA4 was also immunoprecipitated by rabbit anti-EYA4 antibody NBP1-41099, which recognizes an upstream epitope.
Immunohistochemistry: EYA4 Antibody [NBP1-41100]

Immunohistochemistry: EYA4 Antibody [NBP1-41100]

Immunohistochemistry: EYA4 Antibody [NBP1-41100] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-EYA4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-41100]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-41100]

Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-41100] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.

Applications for EYA4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EYA4

EYA4 is thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. It may be involved in development of the eye.

Alternate Names

CMD1J, DFNA10, dJ78N10.1 (eyes absent), EC 3.1.3.48, eyes absent (Drosophila) homolog 4, eyes absent homolog 4, eyes absent homolog 4 (Drosophila)

Entrez Gene IDs

2070 (Human)

Gene Symbol

EYA4

Additional EYA4 Products

Product Documents for EYA4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EYA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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